High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of metformin and gemigliptin was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. Inertsil C18 column C18 (4.6 x 150mm, 5μm) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of metformin and gemigliptin were found to be from 100500 μg/ml of metformin and 1-5μg/ml of gemigliptin. Linear regression coefficient was not more than 0.999.The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of metformin and gemigliptin. LOD and LOQ were found to be within limit. The results obtained on the validation parameters met ICH and USP requirements .it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision. Results: High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of metformin and gemigliptin was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. Inertsil C18 column C18 (4.6 x 150mm, 5μm) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of metformin and gemigliptin were found to be from 100500 μg/ml of metformin and 1-5μg/ml of gemigliptin. Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of metformin and gemigliptin. LOD and LOQ were found to be within limit. The results obtained on the validation parameters met ICH and USP requirements .it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision

Staphylococcal Scalded Skin Syndrome (SSSS) is a remarkable skin disease. The hallmark of SSSS is the peeling off effect on epidermal layer of skin, due to the two exfoliative toxins (ETA & ETB) released by Staph. aureus. Children below 5 years old and neonates, are the main sufferers of SSSS due to devoid of antibodies against the toxins. In adults SSSS, is rarely occurred because of their good immune system, but in immunocompromised patients SSSS can be seen. SSSS can be treated effectively with IV antibiotics, and supportive measures for wound care, prevention of dehydration, pain management can lead to the speedy recovery of SSSS in paediatrics. Prognosis of SSSS in children is excellent, complete resolution of skin observed within 10 days of treatment and mortality rate is also very low. In adults, especially patients with serious comorbid conditions and weak immune system, speedy recovery from SSSS is still challenging

Nanoemulsions of Memecylaene were formulated and evaluated for their anti-inflammatory, antioxidant, and antiangiogenic properties. Several chromatographic methods were used to isolate memecylaene from Memecylon malabaricum leaves.Sunflower oil served as the oily phase, Tween 85 served as the surfactants, and C2H5OH served as the co-surfactant in the sonication process used to create memecylaenenano-emulsions. A zeta potential experiment and droplet size analysis were performed on the nanoemulsion. Studies of stability were carried out, as well as biochemical tests on the nanoemulsions. A zeta potential of - 1.30 mV was determined for the synthesized nanoemulsion with particle sizes ranging from 60.05 nm to 60.50 nm. Embedded Memecylaene in O/W emulsions exhibits enhanced anti-inflammatory and antisecretagogues effects by inhibiting phospholipase (PLA2) enzymes and H +, K+-ATPases, therefore demonstrating enhanced activity. Nitric oxide radicals scavenging activity and DPPH radical scavenging activity were used to evaluate anti-oxidant activity in vitro. Angiogenesis is also inhibited in chick chorioallantoic membrane (CAM) assay, which is an in-vivo model system that is dependent on angiogenesis. Memecylaene-loaded nano-emulsions have the potential to be used as therapeutic agents based on the findings above