Since ages humans have been dependent on plants as one of their primary source of food for survival. Plants are not only responsible for providing food but also they have many unexplored medicinal properties, humans have been able to harness only a very less proportion of its potentials to cure many deadly disease. The current trend of usage of synthetic drugs shifted to natural drugs as the former class of drugs have major side effects which are mostly life threatening, in order to nullify these side effects caused by the drugs to an extent scientists have shifted their focus on natural origin drugs as they are having very less side effects compared to synthetic origin drugs. Out of all the life threatening diseases which humans are facing by humans is diabetics, diabetics is an chronic disorder which is caused by impaired functioning of liver which fails either to produce insulin or reduce its sensitivity towards insulin as a result of which the blood glucose levels will rise than normal range. In traditional system of medicine there are many formulations which have been prescribed by traditional system of medicine practitioners, each and every formulation have its own mode and mechanism of action towards combating diabetes, one such formulation is thetran kottai kudineer which will render its action of anti-diabetics by anti oxidant activity but scientifically underexplored. Thus the present research is focused on the compilation of the standardization parameters carried out as per standards and also its potential anti oxidant activity by DPPH method.KEY WORDS: Thetran kottai kudineer, antioxidant activity, DPPH method.